Use the Standard Curve Calculator to determine the concentration of an unknown sample from its absorbance. This tool is essential for various scientific and industrial applications.
Understanding Standard Curves
Standard curves are graphical representations used in quantitative research to determine the concentration of substances. They are constructed by plotting known concentrations of a substance against the corresponding measurement values (such as absorbance). The resulting line can be used to find the concentration of an unknown sample by measuring its value and comparing it to the curve.
Concentration = (Absorbance - Intercept) / Slope
Variables:
- Concentration is the amount of substance present in the sample (mg/L).
- Absorbance is the measure of the amount of light absorbed by the sample.
- Intercept is the y-intercept of the standard curve equation.
- Slope is the gradient of the standard curve line.
To calculate the concentration, subtract the intercept from the absorbance and then divide by the slope. This method ensures accurate